A Validated Liquid-Liquid Extraction Method with Direct Injection of Hexane for Clopidogrel in Human Plasma Using UltraPerformance Convergence Chromatography (UPC2) and Xevo TQ-S

K E Y W O R D S Convergence chromatography, UPC, tandem quadrupole MS, triple quadrupole MS, liquid-liquid extraction, LLE, clopidogrel A P P L I C AT IO N B E N E F I T S Direct injection of bioanalytical liquid-liquid extracts (LLE) onto UPC2®/MS instrumentation without the need for evaporation and reconstitution. IN T RO DU C T IO N Clopidogrel, shown in Figure 1, is a thienopyridine derivative antiplatelet pro-drug used in the prevention of artherosclerotic events. Following oral administration, the dosed compound undergoes hepatic metabolism to give rise to the active thiol-metabolite and the inactive carboxylic acid metabolite. The inactive metabolite accounts for the majority of circulating clopidogrel-related material in humans, while the active metabolite and unchanged pro-drug are present at very low levels. The mechanism of action is derived from the binding of the active thiol metabolite to cell receptor P2Y12, irreversibly inhibiting the platelet activation process.